کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2180755 1550017 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic interactions among homologous recombination mutants in Candida albicans
ترجمه فارسی عنوان
تعاملات ژنتیکی میان جهش های نوترکیب هومولوگ در کاندیدا آلبیکنس
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


• rad52 and, to a lesser extent, rad51 deletants of C. albicans displayed slow growth and aberrant filamentous morphology.
• We have constructed rad51 rad59, rad52 rad59 and rad51 rad52 strains to analyze genetic interactions between mutations.
• The rad52 mutation is epistatic to rad51 for growth rate, colony morphology, viability, and filamentation ability.
• The rad52 mutation is epistatic to rad51 for the generation of a subpopulation of aberrant yeast and filamentous forms.
• Deletion of RAD59 did not affect growth rate or cell/colony morphology of wild type, rad51 or rad52 strains.

rad52-ΔΔ and, to a lesser extent, rad51-ΔΔ deletants of Candidaalbicans displayed slow growth and aberrant filamentous morphology whereas rad59-ΔΔ mutants, both by growth rate and morphology resembled wild type. In this study, we have constructed pair-wise double deletants to analyze genetic interactions among these homologous recombination (HR) proteins that affect growth and morphology traits. When grown in liquid YPD medium, double mutant rad51-ΔΔ rad59-ΔΔ exhibited growth rates, cell and colony morphologies, and plating efficiencies that were not significantly different from those observed for rad51-ΔΔ. The same was true for rad52-ΔΔ rad59-ΔΔ compared to rad52-ΔΔ. Slow growth and decreased plating efficiency were caused, at least in part, by a decreased viability, as deduced from FUN1 staining. Flow cytometry and microscopic studies of filamentous mutant populations revealed major changes in cell ploidy, size and morphology, whereas DAPI staining identified complex nuclear rearrangements in yeast and filamentous cells. These phenotypes were not observed in the rad59-ΔΔ mutant populations. Our results show that abolishing Rad51 functions induces the appearance of a subpopulation of aberrant yeast and filamentous forms with increased cell size and ploidy. The size of this complex subpopulation was exacerbated in rad52-ΔΔ mutants. The combination of filamentous cell morphology and viability phenotypes was reflected on the colony morphology of the respective mutants. We conclude that the rad52 mutation is epistatic to rad51 for all the morphological traits analyzed. We discuss these results in the light of the several functions of these recombination genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 74, January 2015, Pages 10–20
نویسندگان
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