کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2180897 | 1095241 | 2012 | 8 صفحه PDF | دانلود رایگان |
Numerous polyketides are known from bacteria, plants, and fungi. However, only a few have been isolated from basidiomycetes. Large scale genome sequencing projects now help anticipate the capacity of basidiomycetes to synthesize polyketides. In this study, we identified and annotated 111 type I and three type III polyketide synthase (PKS) genes from 35 sequenced basidiomycete genomes. Phylogenetic analysis of PKS genes suggests that all main types of fungal iterative PKS had already evolved before the Ascomycota and Basidiomycota diverged. A comparison of genomic and metabolomic data shows that the number of polyketide genes exceeds the number of known polyketide structures by far. Exploiting these results to design degenerate PCR primers, we amplified and cloned the complete sequence of armB, a PKS gene from the melleolide producer Armillaria mellea. We expect this study will serve as a guide for future genomic mining projects to discover structurally diverse mushroom-derived polyketides.
► We identified and annotated 114 polyketide synthase (PKS) genes from 35 sequenced basidiomycete genomes.
► The molecular phylogeny of PKS domains was inferred and interpreted from a combined phylogenetic and functional perspective.
► A complete PKS gene from Armillaria mellea was cloned and sequenced.
Journal: Fungal Genetics and Biology - Volume 49, Issue 12, December 2012, Pages 996–1003