کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2180981 1095255 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides
چکیده انگلیسی

The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.


► Expression studies with isoprenoid genes were performed in Mucorcircinelloides.
► Overexpression of the ipi, isoA and carG genes enhanced the carotenoid production.
► The highest production was achieved by the elevation of the copy number of carG.
► Expression of an exogenous β-carotene ketolase yielded xanthophyll-producing strains.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 48, Issue 7, July 2011, Pages 696–703
نویسندگان
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