کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181146 1095274 2009 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cpc1 mediates cross-pathway control independently of Mbf1 in Fusarium fujikuroi
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Cpc1 mediates cross-pathway control independently of Mbf1 in Fusarium fujikuroi
چکیده انگلیسی

The deletion of glnA, encoding the glutamine synthetase (GS), had led to the down-regulation of genes involved in secondary metabolism and up-regulation of cpc1, the cross-pathway control transcription factor. In the present study, a Δcpc1 mutant was created and used for transcriptional profiling by macroarray analysis. Most of the Cpc1 target genes were amino acid biosynthesis genes besides a homologue of the multi-protein bridging factor MBF1 that binds to the yeast Cpc1 homologue GCN4. We show that Δmbf1 mutants exhibit no Cpc1-related phenotype and that both proteins do not interact with each other in Fusarium fujikuroi. Moreover, results presented here suggest that Cpc1 is not responsible for the GS-dependent down-regulation of secondary metabolism and that its role is focused on the activation of amino acid biosynthesis in response to the amino acid status of the cell. Surprisingly, cross-pathway control is repressed by nitrogen limitation in an AreA-dependent manner.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 46, Issue 12, December 2009, Pages 898–908
نویسندگان
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