Spa2 is required for morphogenesis but it is dispensable for pathogenicity in the phytopathogenic fungus Ustilago maydis

The increasing evidence linking regulation of polar growth and pathogenicity in fungi has elicited a significant effort devoted to produce a better understanding of mechanisms determining polarization in pathogenic fungi. Here we characterize in the phytopathogenic basidiomycete Ustilago maydis, the Spa2 protein, a well-known component of polarisome, firstly described in Saccharomyces cerevisiae. U. maydis display a dimorphic switch between budding growth of hapoid cells and filamentous growth of the dikaryon. During yeast growth, a GFP-tagged Spa2 protein localized to distinct growth sites in a cell cycle-specific manner, while during hyphal growth is persistently located to hyphal tips. Deletion of spa2 gene produces rounder budding cells and thicker filaments than wild-type cells, suggesting a role of Spa2 for the determination of the growth area in U. maydis. We also address the connections between Spa2 and the actin- and microtubule-cytoskeleton. We found that the absence of Spa2 does not affect cytoskeleton organization and strikingly, interference with actin filament or microtubule formation does not affect the polar localization of Spa2. In contrast, defects in the small GTPase Rac1 seems to affect the ability of Spa2 to locate to precise sites at the tip cell. Finally, to our surprise, we found that cells defectives in Spa2 function were as pathogenic as wild-type cells.