کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181374 1095292 2008 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of Fusarium graminearum Mes1 reveals roles in cell-surface organization and virulence
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Characterization of Fusarium graminearum Mes1 reveals roles in cell-surface organization and virulence
چکیده انگلیسی

The surfaces of fungal hyphae are mosaics of carbohydrates and cell-surface proteins. Presently, very little is known about the role of these proteins and their organization at the cell surface. Here, we characterize two Fusarium graminearum genes implicated in cell-surface organization, mes1 (FGSG_06680) and pls1 (FGSG_08695). Mes1 is a homologue of mesA, which is required for the formation of stable polarity axes in Aspergillus nidulans. Pls1 encodes a tetraspanin, which belongs to a class of proteins that have been shown to aggregate in membrane rafts along with integrins and other signaling proteins. Our results indicate that Pls1 is dispensable for saprophytic growth and wheat head infection by F. graminearum (a pathogen that does not form appressoria). However, deletion of mes1 reduces sexual and asexual reproduction, severely perturbs the shape of macroconidia and hyphae, alters the pattern of cell wall deposition and the organization of sterol-rich rafts, and attenuates virulence on wheat heads. Our results provide a basis for identifying determinants of fungal virulence that may localize to specialized domains at the cell surface.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 45, Issue 6, June 2008, Pages 933–946
نویسندگان
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