کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181442 1095296 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Circadian rhythmicity during prolonged chemostat cultivation of Neurospora crassa
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Circadian rhythmicity during prolonged chemostat cultivation of Neurospora crassa
چکیده انگلیسی

Following exposure to light and attainment of steady-state in the chemostat, Neurospora was grown in constant conditions of darkness at 25 °C for 6 days. Biomass samples were taken every 4 h for the extraction of RNA and protein, and the state of the circadian clock was assessed by assaying the levels of three rhythmically expressed mRNAs; frequency (frq), antisense frq (qrf) and clock-controlled gene-14 (ccg-14), and by monitoring the clock-controlled rhythm of sporulation. Our results indicate that the Neurospora clock continued to run in the chemostat. This is the longest reported time that Neurospora has been grown in a chemostat in filamentous form and opens up the possibility of studying the response of Neurospora to a range of stimuli in the absence of confounding effects due to; alterations in growth rate, aging, and changing conditions of the growth medium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 44, Issue 8, August 2007, Pages 754–763
نویسندگان
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