کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181891 1095418 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anillin localization suggests distinct mechanisms of division plane specification in mouse oogenic meiosis I and II
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Anillin localization suggests distinct mechanisms of division plane specification in mouse oogenic meiosis I and II
چکیده انگلیسی


• Anillin protein level increases during mouse oocyte maturation.
• Anillin localizes to a broad ring over anaphase I spindles.
• Anillin localizes to a cortical stripe over the meiosis II spindle midzone.
• Division plane specification may differ between mouse meiosis I and II.

Anillin is a conserved cytokinetic ring protein implicated in actomyosin cytoskeletal organization and cytoskeletal-membrane linkage. Here we explored anillin localization in the highly asymmetric divisions of the mouse oocyte that lead to the extrusion of two polar bodies. The purposes of polar body extrusion are to reduce the chromosome complement within the egg to haploid, and to retain the majority of the egg cytoplasm for embryonic development. Anillin's proposed roles in cytokinetic ring organization suggest that it plays important roles in achieving this asymmetric division. We report that during meiotic maturation, anillin mRNA is expressed and protein levels steadily rise. In meiosis I, anillin localizes to a cortical cap overlying metaphase I spindles, and a broad ring over anaphase spindles that are perpendicular to the cortex. Anillin is excluded from the cortex of the prospective first polar body, and highly enriched in the cytokinetic ring that severs the polar body from the oocyte. In meiosis II, anillin is enriched in a cortical stripe precisely coincident with and overlying the meiotic spindle midzone. These results suggest a model in which this cortical structure contributes to spindle re-alignment in meiosis II. Thus, localization of anillin as a conserved cytokinetic ring marker illustrates that the geometry of the cytokinetic ring is distinct between the two oogenic meiotic cytokineses in mammals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 17, Issue 2, March 2015, Pages 98–106
نویسندگان
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