کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181939 1550059 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons
چکیده انگلیسی

The specification of temporal identity within single progenitor lineages is essential to generate functional neuronal diversity in Drosophila and mammals. In Drosophila, four transcription factors are sequentially expressed in neural progenitors (neuroblasts) and each regulates the temporal identity of the progeny produced during its expression window. The first temporal identity is established by the Ikaros-family zinc finger transcription factor Hunchback (Hb). Hb is detected in young (newly-formed) neuroblasts for about an hour and is maintained in the early-born neurons produced during this interval. Hb is necessary and sufficient to specify early-born neuronal or glial identity in multiple neuroblast lineages. The timing of hb expression in neuroblasts is regulated at the transcriptional level. Here we identify cis-regulatory elements that confer proper hb expression in “young” neuroblasts and early-born neurons. We show that the neuroblast element contains clusters of predicted binding sites for the Seven-up transcription factor, which is known to limit hb neuroblast expression. We identify highly conserved sequences in the neuronal element that are good candidates for maintaining Hb transcription in neurons. Our results provide the necessary foundation for identifying trans-acting factors that establish the Hb early temporal expression domain.


► Temporal identity within progenitor lineages is important but understudied.
► Hunchback transcription factor specifies first-born temporal identity in Drosophila and mammalian CNS.
► Precise temporal regulation of hunchback transcription is essential for CNS development.
► We have identified cis-regulatory DNA controlling hunchback temporal expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 12, Issues 1–2, January–February 2012, Pages 11–17
نویسندگان
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