کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181948 1550059 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a conserved cis-acting region driving expression of mouse Eomesodermin to the primitive streak, node, and definitive endoderm
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Identification of a conserved cis-acting region driving expression of mouse Eomesodermin to the primitive streak, node, and definitive endoderm
چکیده انگلیسی

The cis-acting elements that regulate Eomes transcription during embryonic development are largely unknown. Here we identify a conserved cis-acting region (EoIV) located ∼20 kb upstream of the Eomes coding region that faithfully drives reporter expression to sites of Eomes expression during gastrulation. Transgenic EoIV-hsp68-GFP expression was evident in the epiblast of early-streak stage mouse embryos at the site where the primitive streak is initiated. At the mid- and late-streak stages, EoIV-hsp68-GFP expression was found in the streak, node region and definitive endoderm with a particular intensive GFP expression in the node region. At the early head fold stage, GFP was expressed in the node region and the surrounding endoderm. In contrast to earlier reports of Eomes mRNA expression, we confirmed Eomes protein expression in the node of early head fold embryos by immunohistochemistry. In vitro, EoIV-hsp68-GFP expression was activated ES cells differentiating into primitive streak-like progeny in response to Bmp and activin treatment.


► The Eomes gene contains a conserved region (EoIV) at −20 kb.
► EoIV-hsp68-GFP is expressed at sites of Eomes expression during gastrulation.
► EoIV-hsp68-GFP is expressed in primitive streak-like ES cell progeny in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 12, Issues 1–2, January–February 2012, Pages 85–93
نویسندگان
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