کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2182080 | 1095439 | 2010 | 8 صفحه PDF | دانلود رایگان |
We have isolated a Brachyury homologue (Ttu-Bra) from the oligochaete annelid Tubifex tubifex which displays a direct mode of development. Developmental RT-PCR analysis showed that Ttu-Bra transcripts are present in embryos at stages 9–11, 16 and 17, but undetectable at the remaining embryonic stages. Whole-mount in situ hybridization demonstrated that Ttu-Bra is expressed transiently in the third quartette of micromeres, which are located at the prospective stomodaeum (at stages 9–11). The second burst of Ttu-Bra expression occurs at the posterior end of stage 16 embryo that undergoes body elongation. Ttu-Bra-expressing cells, which are organized in a circle at stage 16, become aggregated at the proctodaeum at stage 17. Consistent with the results of the RT-PCR analysis, there is no sign of Ttu-Bra-expressing cells in embryos that undergo gastrulation during stages 12–15.
Journal: Gene Expression Patterns - Volume 10, Issue 6, September 2010, Pages 306–313