کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2182360 | 1095459 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish](/preview/png/2182360.png)
Phospholipase C (PLC) is a key enzyme that generates inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), two second messengers in signal transduction. Phospholipase Cε (PLCE1) is a unique member of the PLC family in that it carries both RasGEF and Ras-associating (RA) domains and may serve as an activator and an effector of small GTPases. Recently, mutations in PLCE1 have been associated with early-onset nephrotic syndrome. We have identified the zebrafish ortholog of PLCE1 and characterized its expression pattern in zebrafish embryos by in situ hybridization. Zebrafish plce1 gene encodes a protein of 2248 amino acids that shares 56% identity with mammalian PLCε. During zebrafish embryonic development, plce1 is expressed in a dynamic pattern in a variety of organs, such as brain, muscle, liver, and pronephric glomeruli. Our results suggest that zebrafish may be used as a model organism to address the function of PLCε during the development of these organs.
Journal: Gene Expression Patterns - Volume 9, Issue 5, June 2009, Pages 282–288