کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2182567 1095471 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos
چکیده انگلیسی

The Hemicentrotus pulcherrimus homologue of nanos (HpNanos), that encodes a protein containing two CCHC zinc finger motifs, was isolated from a gastrula cDNA library. The accumulation of HpNanos mRNA during embryonic development and the spatial expression pattern are reported. Developmental northern blot analysis revealed that HpNanos mRNA markedly accumulated during the blastula stages, and then decreased in abundance at the mesenchyme blastula stage. The second phase of HpNanos mRNA expression occurred during gastrulation, after which the expression returned to a low level. Whole-mount in situ hybridization showed that the HpNanos was exclusively expressed in four to six small micromere-descendant cells at the blastula stage. The expression of HpNanos was restricted to the coelomic pouch, which gives rise to the mesoderm of the ventral surface of the adult rudiment, at the prism stage. These results suggest that HpNanos expression will be instrumental for future analyses of the function of small micromere-descendant cells and of the origin of germ cells during sea urchin development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 6, Issue 5, June 2006, Pages 572–577
نویسندگان
, , , , , , ,