کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2183247 1550298 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comprehensive analysis of TLR4-induced transcriptional responses in interleukin 4-primed mouse macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Comprehensive analysis of TLR4-induced transcriptional responses in interleukin 4-primed mouse macrophages
چکیده انگلیسی

Interferon (IFN)γ and interleukin (IL)-4 are central regulators of T helper 1 (Th1) and T helper 2 (Th2) immune responses, respectively. Both cytokines have a major impact on macrophage phenotypes: IFNγ-priming and subsequent TLR4 activation induces so-called “classically activated” macrophages that are characterized by pronounced pro-inflammatory responses, whereas IL-4-treated macrophages, commonly called “alternatively activated”, are known to develop enhanced capacity for endocytosis, antigen presentation and tissue repair and are generally considered anti-inflammatory. Considering IL-4 as priming rather than activating stimulus, we now compared the TLR4-dependent global gene activation program in IFNγ- versus IL-4-pretreated mouse macrophages, which has rarely been studied so far. Although both cytokines frequently induced opposing effects on gene transcription, the subsequent activation of bone marrow-derived macrophages by lipopolysaccharide (LPS) produced a strong, priming-dependent pro-inflammatory response in both macrophage types. For example, the production of key pro-inflammatory cytokines IL-6 and IL-12 was significantly higher in IL-4- versus IFNγ-primed macrophages and several cytokine genes, including Il19, Ccl17, Ccl22, Ccl24 and Cxcl5, were preferentially induced in “alternatively” primed and LPS activated mouse macrophages. In a subset of genes, including IL12a, IFNγ-priming was actually found to suppress LPS-induced gene expression in a Stat1-dependent manner. Our data suggest that IL-4-priming is not per se anti-inflammatory but generates a macrophage that is “tissue protective” but still capable of mounting a strong inflammatory response after TLR4-dependent activation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 215, Issues 9–10, September–October 2010, Pages 780–787
نویسندگان
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