کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2183765 | 1095589 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polymyxin B increases the depletion of T regulatory cell induced by purinergic agonist
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کلمات کلیدی
PPADSPMBBBGBrilliant Blue GOATPCD25Tregoxidized ATPBzATPIL-2adenosine-5′-triphosphate - آدنوزین 5'-تری فسفاتATP - آدنوزین تری فسفات یا ATPinterleukin 2 - اینترلوکین 2T regulatory cells - سلول های تنظیم کننده ایRegulatory T cells - سلولهای تی تنظیمکنندهNAD - نادانNAD, nicotinamide adenine dinucleotide - نیکوتینامید آدنین دینوکلئوتیدImmune response - پاسخ یا واکنش ایمنیpolymyxin B - پلی مکسین BP2X7 receptor - گیرنده P2X7
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Regulatory T cells (Treg) are important in the development of immune tolerance under normal physiological conditions. However, in pathological situations such as cancer, Treg increases have been correlated with bad prognoses. Treg depletion can be achieved in vitro under several stimuli, including the activation of the purinergic P2X7 receptor. Our aim was to determine whether polymyxin B (PMB), which is a positive modulator of this receptor, could affect mice Treg depletion by ATP and related compounds. For that purpose, we evaluated by flow cytometry changes in Treg populations under several treatments with PMB and/or purinergic agonists and antagonists. We found that both ATP and NAD induce a dose-dependent decrease on the Treg CD4+ CD25+ population. PMB not only potentiated the effect of exogenous ATP and NAD, but also decreased the CD4+ CD25+ population when it was applied alone. While ATP mediated effects are related to the P2X7 receptor, PMB effects appear to be related to another mechanism. We conclude that PMB positively modulates the depletion of the CD4+ CD25+ population of Treg. Therefore PMB could constitute a non-canonical drug with potential use on Treg depletion and cancer treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 217, Issue 3, March 2012, Pages 307-315
Journal: Immunobiology - Volume 217, Issue 3, March 2012, Pages 307-315
نویسندگان
Claudio Cappelli, Ximena López, Yohana Labra, Margarita Montoya, Ricardo Fernández, Mónica Imarai, Juan Luis Rojas, Dante Miranda, Alejandro Escobar, Claudio Acuña-Castillo,