کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2184351 1095831 2016 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Contribution of the C-Terminal Region of a Group II Chaperonin to its Interaction with Prefoldin and Substrate Transfer
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Contribution of the C-Terminal Region of a Group II Chaperonin to its Interaction with Prefoldin and Substrate Transfer
چکیده انگلیسی


• Prefoldin (PFD) interacts with group II chaperonin (CPN) for substrate transfer.
• The flexible C-terminal region of CPN protrudes into the cavity of CPN.
• Truncation of the CPN C-terminal region decreases affinity with PFD.
• Truncation also decreases substrate transfer from PFD and folding by CPN.
• The C-terminal region of CPN is a newly found interaction site with PFD.

Prefoldin is a molecular chaperone that captures an unfolded protein substrate and transfers it to a group II chaperonin. Previous studies have shown that the interaction sites for prefoldin are located in the helical protrusions of group II chaperonins. However, it does not exclude the possibility of the existence of other interaction sites. In this study, we constructed C-terminal truncation mutants of a group II chaperonin and examined the effects of these mutations on the chaperone's function and interaction with prefoldin. Whereas the mutants with up to 6 aa truncation from the C-terminus retained more than 90% chaperone activities for protecting citrate synthase from thermal aggregation and refolding of green fluorescent protein and isopropylmalate dehydrogenase, the truncation mutants showed decreased affinities for prefoldin. Consequently, the truncation mutants showed reduced transfer efficiency of the denatured substrate protein from prefoldin and subsequent chaperonin-dependent refolding. The results clearly show that the C-terminal region of group II chaperonins contributes to their interactions with prefoldin, the transfer of the substrate protein from prefoldin and its refolding.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 428, Issue 11, 5 June 2016, Pages 2405–2417
نویسندگان
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