کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2184588 1095892 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Coilin Displays Differential Affinity for Specific RNAs In Vivo and Is Linked to Telomerase RNA Biogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Coilin Displays Differential Affinity for Specific RNAs In Vivo and Is Linked to Telomerase RNA Biogenesis
چکیده انگلیسی

Coilin is widely known as the protein marker of the Cajal body, a subnuclear domain important to the biogenesis of small nuclear ribonucleoproteins and telomerase, complexes that are crucial to pre-messenger RNA splicing and telomere maintenance, respectively. Extensive studies have characterized the interaction between coilin and the various other protein components of CBs and related subnuclear domains; however, only a few have examined interactions between coilin and nucleic acid. We have recently published that coilin is tightly associated with nucleic acid, displays RNase activity in vitro, and is redistributed to the ribosomal RNA (rRNA)-rich nucleoli in cells treated with the DNA-damaging agents cisplatin and etoposide. Here, we report a specific in vivo association between coilin and rRNA, U small nuclear RNA (snRNA), and human telomerase RNA, which is altered upon treatment with DNA-damaging agents. Using chromatin immunoprecipitation, we provide evidence of coilin interaction with specific regions of U snRNA gene loci. We have also utilized bacterially expressed coilin fragments in order to map the region(s) important for RNA binding and RNase activity in vitro. Additionally, we provide evidence of coilin involvement in the processing of human telomerase RNA both in vitro and in vivo.

Graphical AbstractFigure optionsDownload high-quality image (221 K)Download as PowerPoint slideHighlights
► What is the relationship between coilin and RNAs, specifically telomerase RNA processing?
► We report a specific in vivo association between coilin and rRNA, U snRNA, and telomerase RNA.
► We also show a change in RNA association upon cellular stress by cisplatin and etoposide.
► Additionally, we provide evidence of coilin involvement in telomerase RNA biogenesis.
► We provide evidence of a more operative role of coilin in RNA biogenesis, aside from its function in Cajal body integrity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 425, Issue 4, 22 February 2013, Pages 713–724
نویسندگان
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