کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2184612 1095893 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adaptive Remodelling by FliN in the Bacterial Rotary Motor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Adaptive Remodelling by FliN in the Bacterial Rotary Motor
چکیده انگلیسی


• Adaptive remodelling has been observed in rotary motors for FliM but not for FliN.
• Using an internal fluorescent fusion, we find that remodelling does occur with FliN.
• Remodelling appears to be enhanced by motor switching.
• This work reconciles the apparent mismatch between the activities of FliM and FliN.

Sensory adaptation in the Escherichia coli chemosensory pathway has been the subject of interest for decades, with investigation focusing on the receptors that process extracellular inputs. Recent studies demonstrate that the flagellar motors responsible for cell locomotion also play a role, adding or subtracting FliM subunits to maximise sensitivity to pathway signals. It is difficult to reconcile this FliM remodelling with the observation that partner FliN subunits are relatively static fixtures in the motor. By fusing a fluorescent protein internally to FliN, we show that there is in fact significant FliN remodelling. The kinetics and stoichiometry of FliN in steady state and in adapting motors are investigated and found to match the behaviour of FliM in all respects except for timescale where FliN rates are about 4 times slower. We notice that motor adaptation is slower in the presence of the fluorescent protein, indicating a possible source for the difference. The behaviour of FliM and FliN is consistent with a kinetic and stoichiometric model that contradicts the traditional view of a packed, rigid motor architecture.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 426, Issue 19, 23 September 2014, Pages 3314–3324
نویسندگان
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