کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2185169 1095963 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural Insights into the Novel Diadenosine 5′,5‴-P1,P4-Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Structural Insights into the Novel Diadenosine 5′,5‴-P1,P4-Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv
چکیده انگلیسی

Rv2613c is a diadenosine 5′,5‴-P1,P4-tetraphosphate (Ap4A) phosphorylase from Mycobacterium tuberculosis H37Rv. Sequence analysis suggests that Rv2613c belongs to the histidine triad (HIT) motif superfamily, which includes HIT family diadenosine polyphosphate (ApnA) hydrolases and Ap4A phosphorylases. However, the amino acid sequence of Rv2613c is more similar to that of HIT family ApnA hydrolases than to that of typical Ap4A phosphorylases. Here, we report the crystal structure of Rv2613c, which is the first structure of a protein with ApnA phosphorylase activity, and characterized the structural basis of its catalytic activity. Our results showed that the structure of Rv2613c is similar to those of other HIT superfamily proteins. However, Asn139, Gly146, and Ser147 in the active site of Rv2613c replace the corresponding Gln, Gln, and Thr residues that are normally found in HIT family ApnA hydrolases. Furthermore, analyses of Rv2613c mutants revealed that Asn139, Gly146, and Ser147 are important active-site residues and that Asn139 has a critical role in catalysis. The position of Gly146 might influence the phosphorylase activity. In addition, the tetrameric structure of Rv2613c and the presence of Trp160 might be essential for the formation of the Ap4A binding site. These structural insights into Rv2613c may facilitate the development of novel structure-based inhibitors for treating tuberculosis.

Graphical AbstractFigure optionsDownload high-quality image (274 K)Download as PowerPoint slideResearch Highlights
► Rv2613c is a novel Ap4A phosphorylase.
► The crystal structure of Rv2613c was determined.
► Asn139, Gly146, and Ser147 are important residues for catalytic activity.
► The tetrameric structure might be essential for the formation of the Ap4A binding site.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 410, Issue 1, 1 July 2011, Pages 93–104
نویسندگان
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