کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2185558 | 1095992 | 2010 | 10 صفحه PDF | دانلود رایگان |
The NMR structure of the severe acute respiratory syndrome coronavirus nonstructural protein (nsp) 7 in aqueous solution at pH 6.5 was determined and compared with the results of previous structure determinations of nsp7 in solution at pH 7.5 and in the crystals of a hexadecameric nsp7/nsp8 complex obtained from a solution at pH 7.5. All three structures contain four helices as the only regular secondary structures, but there are differences in the lengths and sequence locations of the four helices, as well as between the tertiary folds. The present study includes data on conformational equilibria and intramolecular rate processes in nsp7 in solution at pH 6.5, which provide further insights into the polymorphisms implicated by a comparison of the three presently available nsp7 structures.
Graphical AbstractFigure optionsDownload high-quality image (159 K)Download as PowerPoint slideResearch highlights
► SARS-CoV nonstructural protein-7 four-helix bundle shows pH-dependent polymorphism.
► The SARS-CoV nsp7 conformation at pH 6.5 shows transient unfolding of helix α4.
Journal: Journal of Molecular Biology - Volume 402, Issue 4, 1 October 2010, Pages 619–628