کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2187017 1096094 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Truncated Cockayne Syndrome B Protein Represses Elongation by RNA Polymerase I
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Truncated Cockayne Syndrome B Protein Represses Elongation by RNA Polymerase I
چکیده انگلیسی

Mutations in the Cockayne syndrome B (CSB) gene result in the human form of Cockayne syndrome. CSB protein has been shown to be a component of RNA polymerase I (Pol I) transcription. In this study, we have analyzed at which step of the transcription cycle CSB influences in vitro transcription by RNA Pol I. We demonstrate that CSB stimulates elongation of RNA Pol I in an ATP-independent manner. Moreover, CSB can be cross-linked to the rDNA promoter and gene-internal sequences. Partial deletion mutants of CSB strongly repress Pol I in vitro transcription, indicating an inhibitory function of truncated CSB. In addition, evidence that mutant CSB inhibits the elongation step of Pol I transcription is presented. Lack of CSB expression does not impair Pol I transcription, showing that CSB is not essential for ribosomal transcription. Our results implicate that repressed Pol I transcription could be one factor contributing to the Cockayne syndrome phenotype.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 382, Issue 2, 3 October 2008, Pages 266–274
نویسندگان
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