کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2187046 1096095 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Upstream Stimulatory Factors Regulate OSCAR Gene Expression in RANKL-Mediated Osteoclast Differentiation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Upstream Stimulatory Factors Regulate OSCAR Gene Expression in RANKL-Mediated Osteoclast Differentiation
چکیده انگلیسی

SummaryReceptor activator of nuclear factor κB ligand (RANKL) induces osteoclast differentiation from hematopoietic precursors via regulation of various transcription factors. Here, we show that upstream stimulatory factors (USFs), which are basic helix–loop–helix leucine zipper transcription factors, are involved in RANKL-mediated osteoclastogenesis and regulation of osteoclast-associated receptor (OSCAR) gene expression, which is an important costimulatory receptor for osteoclast differentiation. USF-1 and USF-2 are expressed in hematopoietic osteoclast precursors and mature osteoclasts. Interestingly, down-regulation of USF-1 by RNA interference or overexpression of a dominant-negative form of USF-1 attenuates osteoclast formation and expression of OSCAR during osteoclastogenesis. Promoter analysis and chromatin immunoprecipitation assays reveal that USFs bind directly to an E-box site in the OSCAR promoter region and activate OSCAR. In addition, USFs interact and cooperate with nuclear factor of activated T cells c1 in regulating OSCAR gene expression. Taken together, our results indicate that USFs serve as modulators in the induction of OSCAR and RANKL-mediated osteoclastogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 383, Issue 3, 14 November 2008, Pages 502–511
نویسندگان
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