کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2187800 1096140 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Critical Role of Mac-1 Sialyl Lewis X Moieties in Regulating Neutrophil Degranulation and Transmigration
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Critical Role of Mac-1 Sialyl Lewis X Moieties in Regulating Neutrophil Degranulation and Transmigration
چکیده انگلیسی

Leukocyte cell surface sialyl Lewis x (sLex) and related epitopes play an important role in cell rolling and adhesion during diapedesis via interaction with E-selectin. Here, we present evidence that Mac-1 (CD11b/CD18, CR-3) is a major neutrophil glycoprotein decorated with sLex and ligation of these carbohydrate moieties by anti-sLex antibody significantly impairs neutrophil functions. First, Western blot analysis shows that both CD11b and CD18 subunit of purified Mac-1 are decorated with sLex moieties. A significant co-localization of CD11b and sLex moieties is observed at neutrophil secondary granules. With stimulation of formyl-Met-Leu-Phe (fMLP), neutrophil surface labeling with anti-sLex antibody follows an identical up-regulation pattern of Mac-1. Second, protein-binding assays indicate that sLex moieties on Mac-1 are critical for binding interaction of Mac-1 to E-selectin. Removal of sLex moieties completely abolishes Mac-1–E-selectin binding. Finally, ligation of Mac-1 sLex by anti-sLex antibody induces a significant degranulation of neutrophil secondary granules at the absence of chemoattractant stimulation. This “dysregulated” degranulation induced by anti-sLex antibody strongly inhibits neutrophil transmigration in response to fMLP. In summary, Mac-1 sLex moieties play a critical role in regulating β2 integrin functions during neutrophil transmigration and degranulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 374, Issue 1, 16 November 2007, Pages 54–63
نویسندگان
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