کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2188360 1096164 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Zinc Finger Proteins ZXDA and ZXDC Form a Complex that Binds CIITA and Regulates MHC II Gene Transcription
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The Zinc Finger Proteins ZXDA and ZXDC Form a Complex that Binds CIITA and Regulates MHC II Gene Transcription
چکیده انگلیسی

The transcription of major histocompatibility complex class II (MHC II) genes depends critically upon the activity of the class II trans-activator (CIITA) protein. We previously described a novel CIITA-binding protein named zinc finger X-linked duplicated family member C (ZXDC) that contributes to the activity of CIITA and the transcription of MHC II genes. Here, we examined the contribution of a closely related family member of ZXDC, the ZXDA protein, to MHC II gene transcription. ZXDA has a domain organization similar to ZXDC, containing ten zinc fingers and a transcriptional activation domain. Knockdown and overexpression of ZXDA demonstrated its importance in the transcriptional activation of MHC II genes. We found that ZXDA and ZXDC can self-associate, and also form a complex with each other. The regions of the two proteins that contain zinc fingers mediate these interactions. Importantly, we found that the ZXDA-ZXDC complex interacts with CIITA, rather than either protein alone. Given our additional finding that ZXDC is present at MHC II promoters in HeLa cells, prior to and after treatment with IFN-γ, it appears that ZXDA and ZXDC form an important regulatory complex for MHC II gene transcription.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 369, Issue 5, 22 June 2007, Pages 1175–1187
نویسندگان
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