کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2188802 1096185 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transition-States in Protein Folding Kinetics: The Structural Interpretation of Φ values
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Transition-States in Protein Folding Kinetics: The Structural Interpretation of Φ values
چکیده انگلیسی

Φ values are experimental measures of the effects of mutations on the folding kinetics of a protein. A central question is what structural information Φ values give about the transition-state of folding. Traditionally, a Φ value is interpreted as representing the ‘nativeness’ of a mutated residue in the transition-state. However, this interpretation is often problematic. We present here a better structural interpretation of Φ values for mutations within a given helix. Our interpretation is based on a simple physical model that distinguishes between secondary and tertiary free energy contributions of helical residues. From a linear fit of the model to experimental data, we obtain two structural parameters: the extent of helix formation in the transition-state, and the nativeness of tertiary interactions in the transition-state. We apply the model to all proteins with well-characterized helices for which more than 10 Φ values are available: protein A, CI2, and protein L. The model is simple to apply to experimental data, captures nonclassical Φ values < 0 or > 1 in these helices, and explains how different mutations at a given site can lead to different Φ values.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 365, Issue 5, 2 February 2007, Pages 1578–1586
نویسندگان
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