کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2189767 | 1096222 | 2006 | 15 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Archaeal RNA Polymerase is Sensitive to Intrinsic Termination Directed by Transcribed and Remote Sequences Archaeal RNA Polymerase is Sensitive to Intrinsic Termination Directed by Transcribed and Remote Sequences](/preview/png/2189767.png)
Archaea are prokaryotes with a single DNA-dependent RNA polymerase (RNAP) that is homologous to, and likely resembles the ancestor of all three eukaryotic RNAPs. In vitro studies have confirmed that initiation by archaeal RNAPs resembles the Pol II system, and we report the first detailed in vitro investigation of archaeal transcription termination. Methanothermobacter thermautotrophicus (M.t.) RNAP is susceptible to intrinsic termination at an intergenic sequence that conforms to a bacterial intrinsic terminator, as well as at bona fide bacterial intrinsic terminators. In contrast to bacterial RNAPs, M.t. RNAP also terminated in response to synthetic and natural oligo-T-rich sequences that were not preceded by sequences with any recognizable potential to form a stable RNA hairpin. Both template topology and temperature influenced the position and extent of termination in vitro, and the results argue that transcription of an upstream sequence can alter the termination response of the archaeal RNAP at a remote downstream sequence.
Journal: Journal of Molecular Biology - Volume 355, Issue 2, 13 January 2006, Pages 196–210