کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2190001 1096231 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Macromolecular Crowding in the Escherichia coli Periplasm Maintains α-Synuclein Disorder
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Macromolecular Crowding in the Escherichia coli Periplasm Maintains α-Synuclein Disorder
چکیده انگلیسی

The natively disordered protein α-synuclein is the primary component of Lewy bodies, the cellular hallmark of Parkinson's disease. Most studies of this protein are performed in dilute solution, but its biologically relevant role is performed in the crowded environment inside cells. We addressed the effects of macromolecular crowding on α-synuclein by combining NMR data acquired in living Escherichia coli with in vitro NMR data. The crowded environment in the E. coli periplasm prevents a conformational change that is detected at 35 °C in dilute solution. This change is associated with an increase in hydrodynamic radius and the formation of secondary structure in the N-terminal 100 amino acid residues. By preventing this temperature-induced conformational change, crowding in the E. coli periplasm stabilizes the disordered monomer. We obtain the same stabilization in vitro upon crowding α-synuclein with 300 g/l of bovine serum albumin, indicating that crowding alone is sufficient to stabilize the disordered, monomeric protein. Two disease-associated variants (A30P and A53T) behave in the same way in both dilute solution and in the E. coli periplasm. These data reveal the importance of approaching the effects of macromolecular crowding on a case-by-case basis. Additionally, our work shows that discrete structured protein conformations may not be achieved by α-synuclein inside cells, implicating the commonly overlooked aspect of macromolecular crowding as a possible factor in the etiology of Parkinson's disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 355, Issue 5, 3 February 2006, Pages 893–897
نویسندگان
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