کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2190217 1096244 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ligand Binding Energy and Enzyme Efficiency from Reductions in Protein Dynamics
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Ligand Binding Energy and Enzyme Efficiency from Reductions in Protein Dynamics
چکیده انگلیسی

Tetrameric rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) binds successively four molecules of its cofactor (NAD+) with affinities of ca 1011 M−1, 109 M−1, 107 M−1, and 105 M−1. The reduction in the dynamics of the protein is greatest upon binding the first NAD+ molecule. Smaller reductions then occur upon binding the second and third NAD+ molecules, and the fourth NAD+ molecule binds without dynamic change. Reduction of the GAPDH dynamics, with consequent improvements in its internal bonding, can account for the increase in NAD+ binding affinity from 105 M−1 to 1011 M−1. Evidence is provided that comparable fractions of the binding energy of other ligands, and of the catalytic efficiency of enzymes, may be derived in the same way.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 355, Issue 4, 27 January 2006, Pages 760–767
نویسندگان
, , ,