کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2190748 | 1097817 | 2011 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ca2+-regulated-cAMP/PKA signaling in cardiac pacemaker cells links ATP supply to demand Ca2+-regulated-cAMP/PKA signaling in cardiac pacemaker cells links ATP supply to demand](/preview/png/2190748.png)
RationaleIn sinoatrial node cells (SANC), Ca2+ activates adenylate cyclase (AC) to generate a high basal level of cAMP-mediated/protein kinase A (PKA)-dependent phosphorylation of Ca2+ cycling proteins. These result in spontaneous sarcoplasmic-reticulum (SR) generated rhythmic Ca2+ oscillations during diastolic depolarization, that not only trigger the surface membrane to generate rhythmic action potentials (APs), but, in a feed-forward manner, also activate AC/PKA signaling. ATP is consumed to pump Ca2+ to the SR, to produce cAMP, to support contraction and to maintain cell ionic homeostasis.ObjectiveSince feedback mechanisms link ATP-demand to ATP production, we hypothesized that (1) both basal ATP supply and demand in SANC would be Ca2+-cAMP/PKA dependent; and (2) due to its feed-forward nature, a decrease in flux through the Ca2+-cAMP/PKA signaling axis will reduce the basal ATP production rate.Methods and resultsO2 consumption in spontaneous beating SANC was comparable to ventricular myocytes (VM) stimulated at 3 Hz. Graded reduction of basal Ca2+-cAMP/PKA signaling to reduce ATP demand in rabbit SANC produced graded ATP depletion (r2 = 0.96), and reduced O2 consumption and flavoprotein fluorescence. Neither inhibition of glycolysis, selectively blocking contraction nor specific inhibition of mitochondrial Ca2+ flux reduced the ATP level.ConclusionsFeed-forward basal Ca2+-cAMP/PKA signaling both consumes ATP to drive spontaneous APs in SANC and is tightly linked to mitochondrial ATP production. Interfering with Ca2+-cAMP/PKA signaling not only slows the firing rate and reduces ATP consumption, but also appears to reduce ATP production so that ATP levels fall. This distinctly differs from VM, which lack this feed-forward basal cAMP/PKA signaling, and in which ATP level remains constant when the demand changes.
► We explored the mechanisms that match ATP supply and demand in pacemaker cells.
► O2 consumption in pacemaker cells is comparable to stimulated ventricular myocytes.
► Reduction in Ca2+-cAMP/PKA signaling in pacemaker cells reduced ATP production.
► Ca2+-cAMP/PKA signaling in pacemaker cells links to ATP consumption and production.
► Matching ATP supply to demand in pacemaker cells differs from ventricular myocytes.
Journal: Journal of Molecular and Cellular Cardiology - Volume 51, Issue 5, November 2011, Pages 740–748