کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2192200 1097886 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interplay of TNF-α and IL-10 in regulating oxidative stress in isolated adult cardiac myocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Interplay of TNF-α and IL-10 in regulating oxidative stress in isolated adult cardiac myocytes
چکیده انگلیسی

Oxidative stress and inflammation are considered to be important factors in the pathogenesis of congestive heart failure subsequent to myocardial infarction. Endogenous TNF-α plays a central role in initiating and sustaining the inflammatory response. IL-10, an anti-inflammatory cytokine, has been shown to antagonize some of the deleterious effects of TNF-α. In this study, we tested whether an imbalance of these two contrasting cytokines leads to increased oxidative stress and cardiac myocyte dysfunction. Isolated adult rat cardiac myocytes were exposed to different concentrations of TNF-α and IL-10 (1–20 ng/ml) alone or in combination. As a positive control, cells were also exposed to H2O2 (100 μÌ) to induce oxidative stress. An exposure to TNF-α (10 ng/ml) caused a significant decrease in both protein and mRNA for manganese superoxide dismutase and catalase, decreased glutathione peroxidase protein, increased intracellular reactive oxygen species and lipid peroxidation, and caused cell injury as measured by creatine kinase release. IL-10 treatment (10 ng/ml) by itself had no effect on any of these parameters, but it prevented all the above listed changes caused by TNF-α. IL-10/TNF-α ratio of lower or higher than 1 was less effective in reducing TNF-α generated oxidative stress. H2O2 treatment increased oxidative stress and cell injury and TNF-α mimicked these effects. This study suggests that a proper balance between IL-10 and TNF-α, rather than any of the individual cytokines is of more physiological importance in mediating oxidative-stress-induced cardiac injury.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 41, Issue 6, December 2006, Pages 1023–1030
نویسندگان
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