کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2192326 1097888 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Treatment with an estrogen receptor-beta-selective agonist is cardioprotective
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Treatment with an estrogen receptor-beta-selective agonist is cardioprotective
چکیده انگلیسی

This study was designed to investigate whether treatment with an estrogen receptor-beta (ER-β)-selective agonist (2,3-bis(4-hydroxyphenyl)-propionitrile, DPN) can provide cardioprotection in female mice lacking endogenous estrogen. To study the effect of ER-β stimulation in ischemia–reperfusion injury, we treated ovariectomized (ovx) female mice with 0.1 mg/kg/day of 17β-estradiol, 0.8 mg/kg/day of DPN, or vehicle for 2 weeks. Isolated hearts were Langendorff perfused for 25 min prior to a 1-min treatment with isoproterenol, followed by 20 min of normothermic global ischemia and 40 min of reperfusion. Left ventricular developed pressure (LVDP) and heart rate were measured. Recovery of function at the end of 40 min of reperfusion was expressed as a percentage of pre-ischemic rate pressure product (RPP = LVDP × heart rate). Hearts from ovx female mice had a significantly lower recovery of LVDP than the hearts from intact female mice (12.4 ± 1.6% vs. 19.6 ± 1.6%, p < 0.05, respectively). Furthermore, hearts from ovx female mice treated with DPN exhibited significantly better functional recovery than hearts from either vehicle-treated ovx female mice (20.1 ± 2.2% vs. 12.4 ± 1.6%, p < 0.05, respectively) or wild type male mice (20.1 ± 2.2% vs. 6.4 ± 0.6%, p < 0.05, respectively). DPN did not increase uterine weight in ovx females compared to vehicle treatment. Gene profiling showed that treatment with DPN resulted in upregulation of a number of protective genes such as heat shock protein 70, the antiapoptotic protein, growth arrest and DNA damage 45 β, and cyclooxygenase 2.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 42, Issue 4, April 2007, Pages 769–780
نویسندگان
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