کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2192338 | 1097888 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Caveolin-1 inhibits matrix metalloproteinase-2 activity in the heart Caveolin-1 inhibits matrix metalloproteinase-2 activity in the heart](/preview/png/2192338.png)
Apart from its ability to degrade extracellular matrix proteins, matrix metalloproteinase-2 (MMP-2) was recently revealed to have targets and actions within the cardiac myocyte. The localization of MMP-2 in caveolae of endothelial cells suggests that caveolin-1 (Cav-1) may play a role in regulating MMP-2. The caveolin scaffolding domain (CSD) of Cav-1 regulates several proteins including those involved with signaling cascades. Whether Cav-1 is responsible for regulating MMP-2 in the heart is unknown. Hearts from Cav-1−/− or Cav-1+/+ mice were isolated and heart extracts or lipid raft enriched membrane fractions were prepared. MMP-2 activity in Cav-1−/− hearts was markedly enhanced when compared with Cav-1+/+ hearts with no changes in MMP-2 protein levels between groups. In contrast, MMP-2 activity and protein level were greatly reduced in lipid raft enriched fractions of Cav-1−/− hearts. Purified CSD inhibited MMP-2 activity in a concentration-dependent manner as assessed using an in vitro degradation assay with a fluorogenic MMP-2 substrate (OmniMMP). These data suggest that Cav-1 plays a role in regulating MMP-2 activity. Cav-1 may thus be a novel mechanism to regulate MMP-2 activity in the heart.
Journal: Journal of Molecular and Cellular Cardiology - Volume 42, Issue 4, April 2007, Pages 896–901