کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2194610 1550575 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hematopoietic activity in putative mouse primordial germ cell populations
ترجمه فارسی عنوان
فعالیت هماتوپوئیدی در جمعیتهای سلولهای ژرمین پیش قاعدگی موش
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


• Heterogeneous expression of the GOF-18ΔPE-GFP Oct3/4 transgene in putative PGCs.
• Putative PGCs express several genes of hematopoietic precursors.
• A subset of the putative PGCs (Oct3/4-GFPweak CD-34weak/high) possesses hematopoietic CFU activity.
• A subset of putative PGCs is not definitively germline determined.

In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta–gonad–mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFPweak CD-34weak/high cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Development - Volume 136, May 2015, Pages 53–63
نویسندگان
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