کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2194707 | 1550584 | 2013 | 19 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The two Tribolium E(spl) genes show evolutionarily conserved expression and function during embryonic neurogenesis The two Tribolium E(spl) genes show evolutionarily conserved expression and function during embryonic neurogenesis](/preview/png/2194707.png)
Tribolium castaneum is a well-characterised model insect, whose short germ-band mode of embryonic development is characteristic of many insect species and differs from the exhaustively studied Drosophila. Mechanisms of early neurogenesis, however, show significant conservation with Drosophila, as a characteristic pattern of neuroblasts arises from neuroectoderm proneural clusters in response to the bHLH activator Ash, a homologue of Achaete–Scute. Here we study the expression and function of two other bHLH proteins, the bHLH-O repressors E(spl)1 and E(spl)3. Their Drosophila homologues are expressed in response to Notch signalling and antagonize the activity of Achaete–Scute proteins, thus restricting the number of nascent neuroblasts. E(spl)1 and 3 are the only E(spl) homologues in Tribolium and both show expression in the cephalic and ventral neuroectoderm during embryonic neurogenesis, as well as a dynamic pattern of expression in other tissues. Their expression starts early, soon after Ash expression and is dependent on both Ash and Notch activities. They act redundantly, since a double E(spl) knockdown (but not single knockdowns) results in neurogenesis defects similar to those caused by Notch loss-of-function. A number of other activities have been evolutionarily conserved, most notably their ability to interact with proneural proteins Scute and Daughterless.
► Tribolium has two E(spl) homologues, E(spl)1 and E(spl)3.
► Tc E(spl)1 and 3 are expressed in the embryonic cephalic and trunk neuroectoderm.
► E(spl) expression depends both on Notch and Ash.
► The E(spl) RNAi phenotype resembles the neurogenic Notch lof phenotype.
► E(spl)1 and 3 antagonize Dm Sc but only E(spl)1 can interact with Dm Da.
Journal: Mechanisms of Development - Volume 130, Issues 4–5, April–May 2013, Pages 207–225