کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2197020 1550949 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulated expression of endothelial lipase in atherosclerosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Regulated expression of endothelial lipase in atherosclerosis
چکیده انگلیسی

Endothelial lipase (EL) is a major determinant of HDL metabolism and associated with the development of atherosclerosis, however the regulated expression of EL in atherosclerosis is unclear. In this study, we investigated EL expression in rat atherosclerosis and explored the potential mechanisms regulating EL expression by employing LPS on Raw264.7 cells in vitro. Rat atherosclerosis model was established fed on high-cholesterol diet (HCD) combined with vitamin D2 (VD). Western blotting and immunochemistry staining revealed that EL expression was increased in the aorta, especially the atherosclerotic lesions in HCD rats. LPS increased EL expression in a time and dose dependent manner in Raw264.7 cells and NFκB inhibitor, PDTC attenuated the effects of LPS on EL. EMSA revealed that LPS stimulated NFκB binding to EL promoter. In summary, EL was upregulated in rat atherosclerosis and LPS stimulates EL expression in vitro through NFκB activation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 315, Issues 1–2, 5 February 2010, Pages 233–238
نویسندگان
, , , , , ,