کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2197311 1550958 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Suppression of bank vole pancreatic islet function by proinflammatory cytokines
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Suppression of bank vole pancreatic islet function by proinflammatory cytokines
چکیده انگلیسی

Bank voles kept in captivity may develop diabetes. We recently characterized β-cell function of pancreatic islets from normal and glucose intolerant/diabetic bank voles. These animals had features of both human type 1 and type 2 diabetes. Cytokines may impair β-cell function in both types of diabetes. Presently, we studied how pancreatic islets isolated from normal, i.e. glucose tolerant bank voles are affected by proinflammatory cytokines in vitro.Islets were exposed to hIL-1β (25 U/ml) alone or in combination with hTNF-α (1000 U/ml) + mIFN-γ (1000 U/ml) for 48 h, whereupon islet functions were assessed.Cytokines markedly reduced insulin gene expression and the (pro)insulin biosynthesis rate, which was accompanied by a profound depletion of the islet insulin content. The cytokines did not affect the culture medium insulin accumulation and the glucose oxidation rate, but caused a modest increase in medium nitrite, an indicator of nitric oxide (NO) generation. Cytokine-induced decrease in islet insulin content was not prevented by the preferential inducible NO synthase inhibitor aminoguanidine.These findings suggest that the reduction in islet insulin content is not attributed to enhanced exocytosis or related to altered glucose metabolism, but is rather due to a decline in insulin production. The suppressive effects of islet functions elicited by cytokines seem to be mediated by an NO-independent mechanism. In relation to previous studies on cytokine effects on islets from various species, the bank vole islets show a pattern which more resembles human islets than rat or murine islets.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 305, Issues 1–2, 16 June 2009, Pages 1–5
نویسندگان
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