کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2197442 | 1098884 | 2009 | 10 صفحه PDF | دانلود رایگان |

TRPM4 is a Ca2+-activated non-selective cation (CAN) channel that functions in cell depolarization, which is important for Ca2+ influx and insulin secretion in pancreatic β-cells. We investigated TRPM4 expression and function in the β-cell lines HIT-T15 (hamster), RINm5F (rat), β-TC3 (mouse), MIN-6 (mouse) and the α-cell line INR1G9 (hamster). By RT-PCR, we identified TRPM4 transcripts in α- and β-cells. Patch-clamp recordings with increasing Ca2+ concentrations resulted in a dose-dependent activation of TRPM4 with the greatest depolarizing currents recorded from hamster-derived cells. Further, Ca2+ imaging experiments revealed that inhibition of TRPM4 by a dominant-negative effect significantly decreased the magnitude of the Ca2+ signals generated by agonist stimulation compared to control cells. The decrease in the [Ca2+]i resulted in reduced insulin secretion. Our data suggest that depolarizing currents generated by TRPM4 are an important component in the control of intracellular Ca2+ signals necessary for insulin secretion and perhaps glucagon from α-cells.
Journal: Molecular and Cellular Endocrinology - Volume 299, Issue 2, 27 February 2009, Pages 194–203