کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2197543 | 1550966 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phosphorylation of estrogen receptor alpha, serine residue 305 enhances activity Phosphorylation of estrogen receptor alpha, serine residue 305 enhances activity](/preview/png/2197543.png)
Upon ligand binding the estrogen receptor alters its conformation, dimerizes, binds to estrogen response elements (EREs), recruits cofactors and initiates the formation of a transcriptional complex. In addition to estradiol binding, hormone receptor activity is modulated by phosphorylation at several key residues. Previous studies have shown that p21-activated kinase-1 (Pak1) and cyclic-AMP dependent protein kinase (PKA) can phosphorylate ERα at serine residue 305. However, the effects of serine 305 phosphorylation on ERα activity have not been fully characterized. To study these effects, ERα S305E and S305A mutants were created to mimic constitutively phosphorylated or un-phosphorylated states, respectively. Using yeast two-hybrid assays we showed that dimerization of ERα S305E was still ligand dependent. However, the capability of dimerization in the presence of estradiol was significantly higher in S305E compared to wild-type ERα. Transactivation assays demonstrated that phospho-mimetic ERα S305E is active in the absence of ligand. Chromatin immunoprecipitation (ChIP) analysis shows a change of in vivo DNA binding in which S305E mutant binds to ERα DNA target sequences and exhibits increased residency in the absence of ligand. We also observed increased cell growth in cells stably transfected with S305E ERα. Thus, we suggest that phosphorylation of S305 does not trigger ERα dimerization but increases binding to target gene promoters, which can lead to increased cell growth in the absence of estradiol. This implies a shift from hormone-induced activation of ERα to activation through phosphorylation, which could confer resistance to hormone based therapies for breast cancer.
Journal: Molecular and Cellular Endocrinology - Volume 295, Issues 1–2, 25 November 2008, Pages 70–78