کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2198000 1550996 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Angiotensin II activates p44/42 MAP kinase partly through PKCɛ in H295R cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Angiotensin II activates p44/42 MAP kinase partly through PKCɛ in H295R cells
چکیده انگلیسی

Using pharmaceutical and overexpression approaches we have previously reported that in H295R cells, (a) angiotensin II (AII) activates PKCɛ, PKCα and p44/42 MAPK pathway, (b) PKCɛ, PKCα and p44/42 MAPK overexpression inhibits AII-induced CYP11B2 gene transcription and (c) overexpression of PKCɛ inhibits CYP11B2 gene transcription through p44/42 MAPK activation [LeHoux, J.G., Dupuis, G., Lefebvre, A., 2001. Control of CYP11B2 gene expression through differential regulation of its promoter by atypical and conventional protein kinase C isoforms. J. Biol. Chem. 276 (11), 8021–8028; LeHoux, J.G., Lefebvre, A., 2006. Novel protein kinase C-epsilon inhibits human CYP11B2 gene expression through ERK1/2 signalling pathway and JunB. J. Mol. Endocrinol. 36 (1), 51–64]. The aim of the present work was to evaluate the physiological role of endogenous PKCɛ and PKCα isoforms in the activation of p44/42 MAPK by AII. A 50% reduction of PKCɛ protein by siRNA-PKCɛ resulted in 35% inhibition of AII-induced p44/42 MAPK activation. Knockdown of PKCɛ stimulated AII-induced CYP11B2 transcription indicating that the PKCɛ is not involved in the activation of CYP11B2 gene expression by AII. Furthermore, knockdown of PKCα enhanced AII-stimulated CYP11B2 transcription without altering p44/42 MAPK indicating that inhibition of AII-stimulated CYP11B2 gene by PKCα does not involve the p44/42 MAPK signalling pathway. These results thus establish that physiologically, PKCɛ and PKCα act through different signalling pathways to inhibit AII-stimulated CYP11B2 gene expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volumes 265–266, February 2007, Pages 121–125
نویسندگان
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