کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2198151 1551004 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization and sex-related seasonal expression of thyroid receptor subtypes in goldfish
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Molecular characterization and sex-related seasonal expression of thyroid receptor subtypes in goldfish
چکیده انگلیسی

The thyroid hormones, acting through the nuclear thyroid receptors (TRs), play important roles in the growth and development of vertebrates. The present study investigated the molecular structure and season-related expression of the TR isoforms in the male and female goldfish pituitary, brain, liver, gonads, gut, heart, and muscle. Based on sequence alignment with other species, the results demonstrate the presence of: (1) a TRα (TRα-1) consisting of 1496 nucleotides encoding a 466 amino acid protein, (2) a novel splice variant of TRα (TRα-2) containing an out-of-frame deletion of 246 nucleotides in the ligand-binding domain consisting of 1251 nucleotides encoding a 378 amino acid protein, (3) a novel transcript resembling TRα, except for non-homology in the hinge region and a premature stop codon prior to the ligand-binding domain (TRα-truncated; 1418 nucleotides, 206 amino acid protein), and (4) TRβ consisting of 1823 nucleotides encoding a 395 amino acid protein. The findings provide the first demonstration of the presence of a truncated TR isoform in non-mammalian vertebrates. In goldfish, the expression patterns for all TRs subtypes were found to be remarkably similar in both male and female, changing significantly before and during reproductive season. The results provide a frame work for better understanding of the functional significance of novel TR forms and TR subtypes in fish and other vertebrates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 253, Issues 1–2, 11 July 2006, Pages 83–95
نویسندگان
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