کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2198360 1551011 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aromatase and estrogen receptors in male reproduction
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Aromatase and estrogen receptors in male reproduction
چکیده انگلیسی

Aromatase is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues. We have demonstrated that mature rat germ cells express a functional aromatase with a production of estrogens equivalent to that of Leydig cells. In humans in addition to Leydig cells, we have shown the presence of aromatase in ejaculated spermatozoa and in immature germ cells. In most tissues, high affinity estrogen receptors, ERα and/or ERβ, mediate the role of estrogens. Indeed, in human spermatozoa, we have successfully amplified ERβ mRNA but the protein was not detectable. Using ERα antibody we have detected two proteins in human immature germ cells: one at the expected size 66 kDa and another at 46 kDa likely corresponding to the ERα isoform lacking exon 1. In spermatozoa only the 46 kDa isoform was present, and we suggest that it may be located on the membrane. In addition, in men genetically deficient in aromatase, it is reported that alterations of spermatogenesis occur both in terms of the number and motility of spermatozoa. All together, these observations suggest that endogenous estrogens are important in male reproduction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 246, Issues 1–2, 26 February 2006, Pages 65–68
نویسندگان
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