کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2199202 | 1099431 | 2007 | 12 صفحه PDF | دانلود رایگان |
The aromatase (ArKO) knockout mouse is estrogen deficient. Our previous analysis revealed apoptosis of dopaminergic neurons in the arcuate nucleus (Arc) and medial preoptic area (MPO) of 1-year-old male ArKO mice. We sought to determine which estrogen receptor (ER) is involved in the anti-apoptotic action of estrogen. Male ArKO (9.5-month-old) mice were treated with 16α-LE2 (ERα-specific agonist) or 8β-VE2 (ERβ-specific agonist). Daily injections (6 weeks) with 16α-LE2 prevented dopaminergic cell death in the Arc of male ArKO mice, with no significant effect of 8β-VE2 treatment. In contrast, 8β-VE2 prevented dopaminergic cell death in the MPO, while 16α-LE2 had no significant effect. Concomitant decreases in Fas and FasL protein levels were found upon 16α-LE2 and 8β-VE2 treatment in the Arc and MPO, respectively. Our results indicate that anti-apoptotic effects of estrogen are ER mediated, and the specific ER subtype involved in regulating apoptosis depends on the particular brain nucleus in question.
Journal: Molecular and Cellular Neuroscience - Volume 36, Issue 2, October 2007, Pages 146–157