کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2199205 | 1099431 | 2007 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: GDNF selectively promotes regeneration of injury-primed sensory neurons in the lesioned spinal cord GDNF selectively promotes regeneration of injury-primed sensory neurons in the lesioned spinal cord](/preview/png/2199205.png)
Axonal regeneration within the CNS fails due to the growth inhibitory environment and the limited intrinsic growth capacity of injured neurons. Injury to DRG peripheral axons induces expression of growth associated genes including members of the glial-derived neurotrophic factor (GDNF) signaling pathway and “preconditions” the injured cells into an active growth state, enhancing growth of their centrally projecting axons. Here, we show that preconditioning DRG neurons prior to culturing increased neurite outgrowth, which was further enhanced by GDNF in a bell-shaped growth response curve. In vivo, GDNF delivered directly to DRG cell bodies facilitated the preconditioning effect, further enhancing axonal regeneration beyond spinal cord lesions. Consistent with the in vitro results, the in vivo effect was seen only at low GDNF concentrations. We conclude that peripheral nerve injury upregulates GDNF signaling pathway components and that exogenous GDNF treatment selectively promotes axonal growth of injury-primed sensory neurons in a concentration-dependent fashion.
Journal: Molecular and Cellular Neuroscience - Volume 36, Issue 2, October 2007, Pages 185–194