کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2199333 | 1099440 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Aquaporin-4 deficiency down-regulates glutamate uptake and GLT-1 expression in astrocytes Aquaporin-4 deficiency down-regulates glutamate uptake and GLT-1 expression in astrocytes](/preview/png/2199333.png)
The role of aquaporin-4 in water transport has been extensively investigated, while little information exists regarding its contribution to astrocytic functions such as the action to glutamatergic transmission. Since aquaporin-4 has been detected widely co-localized with glutamate transporter 1 (GLT-1) and glutamate transporters also present water transport properties, we investigated the regulative role of aquporin-4 on glutamate transporter using primary cultured astrocytes from aquaporin-4 knockout (AQP4−/−) mice. It was demonstrated that lack of aquaporin-4 down-regulated astrocytic expression of GLT-1 but not of glutamate/aspartate transporter (GLAST). The result from [3H]d,l-glutamate uptake analysis showed a lower uptake capability in AQP4−/− astrocytes. Furthermore, MTT and LDH assays indicated less cellular toxicity induced by excessive glutamate in AQP4−/− genotype. These findings provide direct evidences for the first time that aquaporin-4 plays an important role in the function of glutamate transporters. And the present study will improve our understanding of aquaporin-4-glutamanergic biology.
Journal: Molecular and Cellular Neuroscience - Volume 34, Issue 1, January 2007, Pages 34–39