کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2199463 | 1099450 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Persistent activation of protein kinase Cα is not necessary for expression of cerebellar long-term depression
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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![عکس صفحه اول مقاله: Persistent activation of protein kinase Cα is not necessary for expression of cerebellar long-term depression Persistent activation of protein kinase Cα is not necessary for expression of cerebellar long-term depression](/preview/png/2199463.png)
چکیده انگلیسی
Protein kinase Cα (PKCα) plays a major role in the induction of long-term depression (LTD) in a cerebellar Purkinje cell (PC). The sequential activation model for classical PKC states that PKCα translocates to the plasma membrane by binding Ca++ and then becomes fully activated by binding diacylglycerol (DAG), which enables estimation of the activity by monitoring its localization. Here, we performed simultaneous electrophysiological recording and fluorescence imaging in a cultured PC expressing GFP-tagged PKCα. When a PC was depolarized, PKCα transiently translocated to the plasma membrane in a Ca++-dependent manner. Application of membrane permeable DAG or the blocker of DAG lipase prolonged the translocation. These results suggest that the sequential activation model is applicable to PCs. Conjunctive applications of glutamate and depolarization pulse induced LTD, but did not prolong the translocation. Thus, our results imply that persistent activation of PKCα is not necessary for the expression of LTD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 35, Issue 1, May 2007, Pages 38-48
Journal: Molecular and Cellular Neuroscience - Volume 35, Issue 1, May 2007, Pages 38-48
نویسندگان
S. Tsuruno, T. Hirano,