Molecular detection and identification of enteroviruses in children admitted to a university hospital in Greece

Although enteroviral infections occur frequently during childhood, the circulation of particular serotypes has never been studied in Greece. The objectives of the present report were molecular detection and identification of human enteroviruses in children admitted with nonspecific febrile illness or meningitis to a university hospital during a 22-month period. A one-step Real-Time RT-PCR protocol was used for rapid enterovirus detection in genetic material extracted directly from clinical samples, and a sensitive reverse transcription-semi-nested PCR targeting part of the VP1-coding region was used for genotypic identification of the different serotypes. Twenty-one enterovirus strains were detected and identified in 20 stool samples, one cerebrospinal fluid (CSF) sample, one whole blood sample and one throat swab from 21 out of 134 febrile patients (15.7%). Ten strains belonged to Human Enterovirus Species B (HEV-B) (six serotypes) and eleven to HEV-A (four serotypes). Most of the strains were closely associated with virulent strains circulating in Europe and elsewhere. Detection of the emerging pathogen enterovirus 71 for a first time in Greece was particularly important.

► We report on prospective investigation of enterovirus circulation in children.
► Detection and identification were performed by molecular, RT-PCR-based methods.
► The emerging pathogen EV-71 was detected for the first time in Greece.
► Ten strains belonged to Human Enterovirus Species (HEV)-B and eleven to HEV-A.
► Most of the strains were associated with virulent strains circulating worldwide.