کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2199996 | 1099635 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An improved PCR method for gender identification of eagles An improved PCR method for gender identification of eagles](/preview/png/2199996.png)
Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay.
Journal: Molecular and Cellular Probes - Volume 22, Issue 3, June 2008, Pages 184–188