کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2200085 1099641 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of Shiga toxigenic Escherichia coli seropathotypes A and B by multiplex PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Identification of Shiga toxigenic Escherichia coli seropathotypes A and B by multiplex PCR
چکیده انگلیسی

A multiplex PCR assay was developed to identify the six clinically important enterohemorrhagic Escherichia coli (EHEC) serotypes classified in seropathotypes A and B and to differentiate these from Shiga toxigenic E. coli. The assay simultaneously detects genes for Shiga toxin (stx) and intimin (eae), including allelic variants of both genes, 16S internal amplification control, as well as unique sequences in the wzx genes that are specific for serotypes O157, O26, O111, O103, O121 and O145. PCR analysis of 40 representative strains showed that the assay correctly identified the virulence genes, if present, and the respective O antigen type of all the strains, including some atypical EHEC, as well as enteropathogenic E. coli and E. coli strains examined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 21, Issue 4, August 2007, Pages 308–311
نویسندگان
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