کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2200159 1099647 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
AFLP fingerprinting for the analysis of genetic diversity within Necator americanus
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
AFLP fingerprinting for the analysis of genetic diversity within Necator americanus
چکیده انگلیسی

In the present study, we utilised the method of AFLP to screen for genetic variation within and among individuals of the blood-feeding human hookworm Necator americanus (Nematoda) from Africa, Asia and South America. A total of 45 adult worms (i.e. 20 from Ghana, 16 from Colombia and 9 from Nepal) were subjected to analysis using the restriction enzyme/primer combination HindIII+AG/BglII+AC. Cluster analysis divided N. americanus into multiple, genetically distinct groups, consistent with previous findings using ribosomal and mitochondrial DNA data sets. The results demonstrated the usefulness of AFLP fingerprinting for establishing genetic variation within N. americanus and reinforce its applicability to other parasitic helminths of human and/or veterinary health importance.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 20, Issue 5, October 2006, Pages 317–321
نویسندگان
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