کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2201374 1100015 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New evidences on Tau–DNA interactions and relevance to neurodegeneration
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
New evidences on Tau–DNA interactions and relevance to neurodegeneration
چکیده انگلیسی

Tau is mainly distributed in cytoplasm and also found to be localized in the nucleus. There is limited data on DNA binding potential of Tau. We provide novel evidence on nicking of DNA by Tau. Tau nicks the supercoiled DNA leading to open circular and linear forms. The metal ion magnesium (a co-factor for endonuclease) enhanced the Tau DNA nicking ability, while an endonuclease specific inhibitor, aurinetricarboxylic acid (ATA) inhibited the Tau DNA nicking ability. Further, we also evidenced that Tau induces B–C–A mixed conformational transition in DNA and also changes DNA stability. Tau–scDNA complex is more sensitive to DNAse I digestion indicating stability changes in DNA caused by Tau. These findings indicate that Tau alters DNA helicity and integrity and also nicks the DNA. The relevance of these novel intriguing findings regarding the role Tau in neuronal dysfunction is discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 57, Issue 1, August 2010, Pages 51–57
نویسندگان
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